Species specificity:

human, mouse, rat, pig, swine

Positive WB detected in:

HEK-293 cells, pig liver tissue

Positive IP detected in:

HEK-293 cells

Positive IHC detected in:

human liver cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HeLa cells

Positive FC detected in:

HepG2 cells

Recommended dilution:

WB : 1:2000-1:16000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:325-1:1300

IF : 1:50-1:500