Agilent安捷伦 Pfu Turbo DNA Polymerase 高保真聚合酶 600250/600600/600255 First Generation Polymerases - Details & Specifications

Stratagene was the first to commercialize Pfu in 1991. Since that time, many improvements have been added to native Pfu through molecular engineering. The resulting products have delivered greater performance including increased robustness, yield, fidelity, speed, length, and economy.

See PfuUltra II Fusion HS DNA Polymerase  or Herculase II Fusion DNA Polymerase  product pages for more information about Agilent’s next generation PCR polymerases.

In The Beginning There Was Native Pfu DNA Polymerase

• Pfu exhibits the lowest error rate of any thermostable DNA polymerase

• Corrects nucleotide misincorporation errors with 3’ -> 5’ exonuclease activity (proofreading)

• Cloned Pfu eliminates smearing and unwanted background

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' -> 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products which are ideal for cloning into blunt-ended vectors (see StrataClone ). Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Cloned Pfu DNA Polymerase

• Available with an alternative detergent (AD) for greater economy

The cloned version of Pfu allows for the manufacturing of ultrapure Pfu DNA Polymerase which virtually eliminates smearing and unwanted background artifacts while providing consistent performance and supply.

Performance Equal with Economy Greater Offer Polymerases

Agilent’s AD polymerases feature a reformulated buffer system that allows greater control and savings of the production costs associated with the product. The savings are then passed on to you. AD polymerases have been tested with a wide variety of targets and all perform with equal or better performance as our original polymerases with no protocol changes required.

Improving upon Native

 Pfu with PfuTurbo DNA Polymerase

• nhances overall PCR performance (yield and length) by preventing “dUTP poisoning”

• Provides higher yields and length than native or cloned Pfu

• Available in AD, hotstart, and hotstart master mix

PfuTurbo DNA Polymerase, a combination of cloned Pfu and ArchaeMaxx, was the first high fidelity polymerase to overcome dUTP poisoning through the addition of ArchaeMaxx Factor. By overcoming dUTP poisoning, PfuTurbo is able to deliver higher yields and length than were previously achieved.

ArchaeMaxx PCR Enhancing Factor

 ArchaeMaxx, developed by and exclusive to Stratagene, eliminates PCR inhibition due to the inevitable accumulation of dUTP during high fidelity PCR. dUTP accumulation is a result of dCTP deamination during PCR and inhibits archaeal proofreading DNA polymerases limiting their efficiency. ArchaeMaxx functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate resulting in greatly enhanced overall PCR performance.

Rising to New Levels with PfuUltra DNA Polymerase

• Provides the most accurate copies of DNA possible through PCR

• Includes ArchaeMaxx to increase yields and length

• Available in AD, hotstart, and master mix

The PfuUltra DNA polymerase formulation features a genetically engineered mutant of Pfu DNA polymerase and the ArchaeMaxx polymerase-enhancing factor. Featuring an error rate three-fold lower than PfuTurbo DNA Polymerase and 18-fold lower than Taq DNA Polymerase, PfuUltra DNA Polymerase is the highest fidelity enzyme available in the world. Amplification of a 500-bp fragment using PfuUltra DNA polymerase results in errors in less than 0.5% of full-length PCR products, making PfuUltra the ideal enzyme when accurate copies are your primary concern.

A New Generation of Pfu-Based Polymerase